Product Description:

FGF-10 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-10 is most related to KGF/FGF-7, and is expressed during the development and, preferentially, in adult lungs. It signals through the FGFR 2b.

Technical Specifications:

• Species: Human
• Expression: E. coli Cell Expressed
• Activity: Typically ≤0.5 ng/ml ED50
• Purity: ≥95%
• Endotoxin: <1.0 EU/μg
• Molecular Mass: 19.3 kDa
• Formulation: 5mM Sodium Phosphate pH 7.4 + 80 mM NaCl
• Country of Origin: USA

Purity Confirmation:

This was determined by SDS-PAGE gel and HPLC analysis.

Activity Assay:

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg.

AA Sequence:

Reconstitution Buffer:

Centrifuge the vial prior to opening. 5mM Sodium Phosphate, pH 7.4 to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage:

For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% HSA) and store in working aliquots at -20°C to -80°C.