Product Description:

• Endotoxin-free
• Animal-derived product free
• Available in Bulk
• Lyophilized and Carrier Free (CF)

Xeno-free TGF beta 1^HuXp is expressed from human 293 cells as a mature disulfide-linked homodimer with an apparent molecular mass of 25 kDa. Tests indicate that TGF beta 1 is as efficient at inducing IL-17 expression in Th17 cells, as cytokine purified from human platelets. This product is produced in a human cell expression system with serum-free, chemically defined media.

Technical Specifications:

• Species: Human
• Expression: HEK293 Cell Expressed
• Activity: Typically ≤0.5 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Molecular Mass: 25 kDa, homodimer, nonglycosylated
• Formulation: 50 mM NaOAc pH 4.5

Purity Confirmation:

The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

Activity Assay:

The activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2)..

Reconstitution Buffer:

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).