ProductDescription:
RecombinantHumanTransferrinisauniversalironcarriercapableofdeliveringirontocells.Each80kDAtransferrinmoleculecancarrytwoironionsintheferricform.RecombinantHumanTransferrinismanufacturedanimalfreeinaGMPfacilityandcanbeutilizedtoincreasethequantityandqualityofawiderangeofcelltypes.Transferrinisalsoassociatedwiththeinnateimmunesystem,sinceitsbindingofironcancreatedecreasedlevelsoffreeironandimpedebacterialsurvival.Theactivityisequivalenttohumanserumholotransferrin.
TechnicalSpecifications:
- Species:Human
- Expression:OryzaSativa
- Purity:≥95%
- Endotoxin:<1.0EU/μg
- MolecularMass: 80.0kDa
- Formulation:SodiumPhosphateandSodiumChloride,pH7.0
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgel.
ActivityAssay:
BIOLOGicalactivityverifiedbycomparisonagainstnativehumantransferrinintheantibodyproductionofhybridomacellsinserum-freesupplementedmedia.
AASequence:
VPDKTVRWC | AVSEHEATK | CQSFRDHMK |
SVIPSDGPS | VACVKKASY | LDCIRAIAA |
NEADAVTLD | AGLVYDAYL | APNNLKPVV |
AEFYGSKED | PQTFYYAVA | VVKKDSGFQ |
MNQLRGKKS | CHTGLGRSA | GWNIPIGLL |
YCDLPEPRK | PLEKAVANF | FSGSCAPCA |
DGTDFPQLC | QLCPGCGCS | TLNQYFGYS |
GAFKCLKDG | AGDVAFVKH | STIFENLAN |
KADRDQYEL | LCLDNTRKP | VDEYKDCHL |
AQVPSHTVV | ARSMGGKED | LIWELLNQA |
QEHFGKDKS | KEFQLFSSP | HGKDLLFKD |
SAHGFLKVP | PRMDAKMYL | GYEYVTAIR |
NLREGTCPE | APTDECKPV | KWCALSHHE |
RLKCDEWSV | NSVGKIECV | SAETTEDCI |
AKIMNGEAD | AMSLDGGFV | YIAGKCGLV |
PVLAENYNK | SDNCEDTPE | AGYFAIAVV |
KKSASDLTW | DNLKGKKSC | HTAVGRTAG |
WNIPMGLLY | NKINHCRFD | EFFSEGCAP |
GSKKDSSLC | KLCMGSGLN | LCEPNNKEG |
YYGYTGAFR | CLVEKGDVA | FVKHQTVPQ |
NTGGKNPDP | WAKNLNEKD | YELLCLDGT |
RKPVEEYAN | CHLARAPNH | AVVTRKDKE |
ACVHKILRQ | QQHLFGSNV | TDCSGNFCL |
FRSETKDLL | FRDDTVCLA | KLHDRNTYE |
KYLGEEYVK | AVGNLRKCS | TSSLLEACT |
FRRP |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstitutein1xPBStoaconcentrationof5-200mg/Ldependingoncelllineofinterest.Donotvortex.
Storage:
Storeinworkingaliquotsat-20°Cto-80°C.