ProductDescription:
MembersoftheHedgehog(Hh)familyarehighlyconservedproteinsthatarewidelyrepresentedthroughouttheanimalkingdom.ThethreeknownmammalianHhproteins,Sonic(Shh),Desert(Dhh)andIndian(Ihh),arestructurallyrelated,andshareahighdegreeofaminoacidsequenceidentity(e.g.ShhandIhhare93%identical).TheBIOLOGicallyactiveformofeachHhmoleculeisobtainedbyautocatalyticcleavageoftheirprecursorproteins,andeachcorrespondstoapproximatelyonehalfoftheN-terminalportionoftheprecursormolecule.AlthoughHhproteinshaveuniqueexpressionpatternsanddistinctbiologicalroleswithintheirrespectiveregionsofsecretion,theyusethesamesignalingpathwayandcanbesubstitutedforoneanotherinexperimentalsystems.RecombinantE.coli-derivedHumanSonicHedgehogisa20.0kDaproteinconsistingof176aminoacidresidues,includinganN-terminalIle-Val-Ilesequencesubstitutedforthenaturallyoccurring,chemicallymodified,Cysresidue.
TechnicalSpecifications:
- Species:Human
- Expression:E.coliCellExpressed
- Activity:Typically0.8-1.0μg/mlED50
- Purity:≥98%
- Endotoxin:<1.0EU/μg
- MolecularMass:20.0kDa
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
DeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyC3H/10T1/2(CCL-226)cells.
AASequence:
| IVIGPGRGFG | KRRHPKKLTP | LAYKQFIPNV |
| AEKTLGASGR | YEGKISRNSE | RFKELTPNYN |
| PDIIFKDEEN | TGADRLMTQR | CKDKLNALAI |
| SVMNQWPGVK | LRVTEGWDED | GHHSEESLHY |
| EGRALDITTS | DRDRSKYGML | ARLAVEAGFD |
| WVYYESKAHI | HCSVKAENSV | AAKSGG |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
