ProductDescription:
CD40,amemberoftheTNFreceptorfamily,isacellsurfaceproteinexpressedonBcells,dendriticcells,monocytes,thymicepithelialcellsand,atlowlevels,onTcells.SignalingthoughCD40playsanimportantroleintheproliferationanddifferentiationofBcells,andiscriticalforimmunoglobulin(Ig)classswitching.Themembrane-anchoredCD40-LigandisexpressedalmostexclusivelyonactivatedCD4+Tlymphocytes.FailuretoexpressCD40Lleadsto“immunodeficiencywithhyper-IgM”,adiseasecharacterizedbyfailuretoproduceIgG,IgAandIgE.ThehumanCD40Lgenecodesfora261aminoacidtypeIItransmembraneprotein,whichcontainsa22aminoacidcytoplasmicdomain,a24aminoacidtransmembranedomain,anda215aminoacidextracellulardomain.
TechnicalSpecifications:
- Species:Human
- Expression:E.coliCellExpressed
- Activity:Assay1:N/A
- Assay2:Typically5-10ng/mLED50
- Purity:≥98%
- Endotoxin:<1.0EU/μg
- MolecularMass:16.3kDa
- Formulation:10mMSodiumPhosphate,pH7.5
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis
ActivityAssay:
Assay#1:DeterminedbythestimulationofIL-12inductionbyhumanPBMCs.
Assay#2:Determinedbythedose-dependentstimulationofIL-8productionbyhumanPBMC.TheexpectedED50forthiseffectis5-10ng/ml.
Note:ResultsmayvarywithPBMCdonors.
AASequence:
| MQKGDQNPQI | AAHVISEASS | KTTSVLQWAE |
| KGYYTMSNNL | VTLENGKQLT | VKRQGLYYIY |
| AQVTFCSNRE | ASSQAPFIAS | LWLKSPGRFE |
| RILLRAANTH | SSAKPCGQQS | IHLGGVFELQ |
| PGASVFVNVT | DPSQVSHGTG | FTSFGLLKL |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstitutein5-10mMSodiumPhosphate,pH7.5toaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
