ProductDescription:
PDGFsaremitogenicduringearlydevelopmentalstages,drivingtheproliferationofundifferentiatedmesenchymeandsomeProgenitorpopulations.Duringlatermaturationstages,PDGFsignalinghasbeenimplicatedintissueremodelingandcellulardifferentiation,andininductiveeventsinvolvedinpatterningandmorphogenesis.Inadditiontodrivingmesenchymalproliferation,PDGFshavebeenshowntodirectthemigration,differentiationandfunctionofavarietyofspecializedmesenchymalandmigratorycelltypes,bothduringdevelopmentandintheadultanimal.PDGFplaysaroleinembryonicdevelopment,cellproliferation,cellmigration,andangiogenesis.PDGFisarequiredelementincellulardivisionforfibroblast,atypeofconnectivetissuecell.PDGFisalsoknowntomaintainproliferationofoligodendrocyteprogenitorcells.Platelet-derivedgrowthfactorsubunitBisalsoknownasPDGFB,FLJ12858,PDGF2,SIS,SSV,c-sis,isamemberoftheplatelet-derivedgrowthfactorfamily.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Activity:Assay1:Typically0.02-0.6μg/mL
- Assay2:Typically1-10ng/mL
- Purity:>90%
- Endotoxin:<1.0EU/μg
- MolecularMass:25kDa
- Formulation:AcetonitrileandTFA
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGE.
ActivityAssay:
Assay#1:MeasuredbyitsbindingABIlityinafunctionalELISA.
Assay#2:Thebio-activitywasdeterminedbydose-dependentstimulationoftheproliferationofmouse3T3cells.
ReconstitutionBuffer:
SeeCertificateofAnalysisforreconstitutioninstructionsandspecificconcentrations.
Storage:
LyophilizedProteinshouldbestoredat-20°orlowerforlongtermstorage.Uponreconstitution,workingaliquotsshouldbestoredat-20°or-70°.Avoidrepeatedfreeze-thawcycles.