ProductDescription:
Neurturinisadisulfide-linkedhomodimerneurotrophicfactorstructurallyrelatedtoGDNF,artemin,andpersephin.Theseproteinsbelongtothecysteine-knotfamilyofgrowthfactorsthatassumestabledimericstructures.Neurturinsignalsthroughamulticomponentreceptorsystem,composedofRETandoneoffourGFRα(α1-α4)receptors.NeurturinpromotesthedevelopmentandsurvivalofsympatheticandsensoryneuronsbysignalingthroughareceptorsystemcomposedofRETandGFRα2.Thefunctionalformofhumanneurturinisadisulfide-linkedhomodimer,oftwo11.8kDapolypeptidemonomers(204totalaminoacidresidues).Eachmonomercontainssevenconservedcysteineresidues,oneofwhich(Cys69)isusedforinter-chaindisulfidebridging,andtheothersareinvolvedintheintramolecularringformationknownasthecysteineknotconfiguration.
TechnicalSpecifications:
- Species:Human
- Expression:E.coliCellExpressed
- Activity:Typically≤0.1ng/mLED50
- Purity:≥98%
- Endotoxin:<1.0EU/μg
- MolecularMass:11.8kDa,homodimer
- Formulation:10mMSodiumCitrate,pH4.0.
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
HumanNeurturinataconcentrationof100ng/mlwillsupportthesurvivalof65%ofnewbornratsympatheticneurons.
AASequence:
| ARLGARPCGL | RELEVRVSEL | GLGYASDETV |
| LFRYCAGACE | AAARVYDLGL | RRLRQRRRLR |
| RERVRAQPCC | RPTAYEDEVS | FLDAHSRYHT |
| VHELSARECA | CV |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
