ProductDescription:
Interleukin15isacytokinewithstructuralsimilaritytoIL-2.LikeIL-2,IL-15bindstoandsignalsthroughtheIL-2/IL-15betachain(CD122)andthecommongammachain(gamma-C,CD132).IL-15issecretedbymononuclearphagocytes(andsomeothercells)followinginfectionbyvirus(es).Thiscytokineinducescellproliferationofnaturalkillercells;cellsoftheinnateimmunesystemwhoseprincipalroleistokillvirallyinfectedcells.Interleukin15(IL-15)regulatesTandnaturalkiller(NK)cellactivationandproliferation.SurvivalsignalsthatmaintainmemoryTcellsintheabsenceofantigenareprovidedbyIL-15.ThiscytokineisalsoimplicatedinNKcelldevelopment.Inrodentlymphocytes,IL-15preventsapoptosisbyinducinganapoptosisinhibitor,BCL2L1/BCL-x(L).IL-15hasbeenshowntoenhancetheanti-tumorimmunityofCD8+Tcellsinpre-clinicalmodels.AphaseIclinicaltrialtoevaluatethesafety,dosing,andanti-tumorefficacyofIL-15inpatientswithmetastaticmelanomaandrenalcellcarcinoma(kidneycancer)hasbeguntoenrollpatientsattheNationalInstitutesofHealth.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Activity:Typically<0.5ng/mLED50
- Purity:>95%
- Endotoxin:<1.0EU/μg
- MolecularMass:12.8kDa
- Formulation:PBS,pH7.4
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGE.
ActivityAssay:
Determinedbythedose-dependentstimulationoftheproliferationofCTLL-2cells.
ReconstitutionBuffer:
SeeCertificateofAnalysisforreconstitutioninstructionsandspecificconcentrations.
Storage:
LyophilizedProteinshouldbestoredat-20°orlowerforlongtermstorage.Uponreconstitution,workingaliquotsshouldbestoredat-20°or-70°.Avoidrepeatedfreeze-thawcycles.
