ProductDescription:
Neuregulin/Heregulinisafamilyofstructurallyrelatedpolypeptidegrowthfactorsderivedfromalternativelysplicedgenes(NRG1,NRG2,NRG3andNRG4).Todate,thereareover14solubleandtransmembraneproteinsderivedfromtheNRG1gene.ProteolyticprocessingoftheextracellulardomainofthetransmembraneNRG1isoformsreleasessolublegrowthfactors.HRG1-β1containsanIgdomainandanEGF-likedomain;thelatterisnecessaryfordirectbindingtoreceptortyrosinekinaseserb3anderb4.Thisbindinginduceserb3anderb4heterodimerizationwitherb2,stimulatingintrinsickinaseactivitythatleadstotyrosinephosphorylation.AlthoughHRG1-β1’sBIOLOGicaleffectsarestillunclear,ithasbeenfoundtopromotemotilityandinvasivenessofbreastcancercells,whichmayalsoinvolveup-regulationofexpressionandfunctionoftheautocrinemotility-promotingfactor(AMF).RecombinantHumanHeregulinβ-1(HRG1-B1)isa7.5kDapolypeptideconsistingofonlytheEGFdomainofheregulinβ-1(65aminoacidresidues).
TechnicalSpecifications:
- Species:Human
- Expression:E.coliCellExpressed
- Activity:Typically≤0.5ng/mLED50
- Purity:>98%
- Endotoxin:<1.0EU/μg
- MolecularMass:7.5kDa
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
Determinedbythedose-dependentstimulationoftheproliferationofhumanMCF-7cells.
AASequence:
| SHLVKCAEKE | KTFCVNGGEC | FMVKDLSNPS |
| RYLCKCPNEF | TGDRCQNYVM | ASFYKHLGIE |
| FMEAE |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
