ProductDescription:
GDNFisadisulfide-linked,homodimericneurotrophicfactorstructurallyrelatedtoArtemin,NeurturinandPersephin.Theseproteinsbelongtothecysteine-knotsuperfamilyofgrowthfactorsthatassumestabledimericproteinstructures.GDNFsignalsthroughamulticomponentreceptorsystem,composedofaRETandoneofthefourGFRα(α1-α4)receptors.GDNFspecificallypromotesdopamineuptakeandsurvival,andmorphologicaldifferentiationofmidbrainneurons.UsingaParkinson’sdiseasemousemodel,GDNFhasbeenshowntoimproveconditionssuchasbradykinesia,rigidity,andposturalinstABIlity.ThefunctionalhumanGDNFligandisadisulfide-linkedhomodimerconsistingoftwo15kDapolypeptidechainscalledmonomers.Eachmonomercontainssevenconservedcysteineresidues,includingCys-101,whichisusedforinter-chaindisulfidebridging,andothersthatareinvolvedintheintramolecularringformationknownasthecysteineknotconfiguration.
TechnicalSpecifications:
- Species:Human
- Expression:E.coliCellExpressed
- Activity:Typically1.0-10.0ng/mLED50
- Purity:>98%
- Endotoxin:<1.0EU/μg
- MolecularMass:30.4kDa,homodimeric
- Formulation:10mMSodiumCitrate+150mMNaCl
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis
ActivityAssay:
DeterminedbyacellproliferationassayusingSH-SY5Ycells.
AASequence:
| MSPDKQMAVL | PRRERNRQAA | AANPENSRGK |
| GRRGQRGKNR | GCVLTAIHLN | VTDLGLGYET |
| KEELIFRYCS | GSCDAAETTY | DKILKNLSRN |
| RRLVSDKVGQ | ACCRPIAFDD | DLSFLDDNLV |
| YHILRKHSAK | RCGCI |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
