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Other_recombinant_protein >
HumanZyme/DKK-1 (Dickkopf-related protein-1)/HZ-7282/50 µg
ProductDescription:
DKK-1isamemberoftheDKKproteinfamilywhichalsoincludesDKK-2,DKK-3andDKK-4.DKK-1wasoriginallyidentifiedasaXenopushead-formingmoleculethatbehavesasanantagoNISTforWntsignaling.SubsequentstudieshaveshownthatDKK-1andDKK-4playanimportantregulatoryroleintheWnt/β-cateninsignalingpathwaybyforminginhibitorycomplexeswithLDLreceptor-relatedproteins5and6(LRP5andLRP6),whichareessentialcomponentsoftheWnt/β-cateninsignalingsystem.LPR5andLPR6aresingle-passtransmembraneproteinsthatappeartoactasco-receptorsforWntligandsinvolvedintheWnt/β-cateninsignalingcascade.IthasbeensuggestedthatbyinhibitingWnt/β-cateninsignaling,whichisessentialforposteriorpatterninginvertebrates,DKK-1permitsanteriordevelopment.ThisnotionissupportedbythefindingthatmicedeficientofDKK-1expressionlackheadformationanddieduringembryogenesis.MaturehumanDKK-1expressedinHEK293cellsisa35-40kDaglycoproteincontaining235aminoacidresidues.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Purity:≥97%
- Endotoxin:<1.0EU/μg
- MolecularMass:25.8kDa
- Formulation:1xPBS,pH7.4
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
DeterminedbyitsABIlitytoinhibittheproliferationofHCT116colorectalcarcinomacells.Approximately40%growthinhibitionwasachievedataDKK-1concentrationof200ng/ml.
AASequence:
TLNSVLNSN | IKNLPPPLGG | AAGHPGSAVS |
AAPGILYPGG | NKYQTIDNYQ | PYPCAEDEEC |
GTDEYCASPT | RGGDAGVQIC | LACRKRRKRC |
MRHAMCCPGN | YCKNGICVSS | DQNHFRGEIE |
ETITESFGND | HSTLDGYSRR | TTLSSKMYHT |
KGQEGSVCLR | SSDCASGLCC | ARHFWSKICK |
PVLKEGQVCT | KHRRKGSHGL | EIFQRCYCGE |
GLSCRIQKDH | HQASNSSRLH | TCQRH |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.