ProductDescription:
CTGFisamemberoftheCCNfamilyofsecretedcysteine-richregulatoryproteins,andisthemajormitogenicandchemoattractantproteinproducedbyumbilicalveinandvascularendothelialcells.CTGFstimulatestheproliferationanddifferentiationofchondrocytes,inducesangiogenesis,promotescelladhesionoffibroblasts,endothelialandepithelialcells,andbindstoIGF,TGFβ1andBMP-4.CTGFiscomprisedoffourdistinctstructuraldomains(modules),whichareidentifiedasIGFbindingprotein(IGFBP),vonWillebrandFactorC(VWFC),thrombospondintype-I(TSPtype-I),andC-terminalcysteineknot-like(CTCK)domains.FulllengthCTGFcanbeproteolyticallycleavedincertaintissuestoyieldN-terminaltruncatedisoforms,which,dependingontheisoform,containonlytheTSPtype-IandCTCKdomainsorcontainonlytheCTCKdomain.
TechnicalSpecifications:
- Species:Human
- Expression:E.coliCellExpressed
- Activity:Typically1.0-2.0μg/mLED50
- Purity:>95%
- Endotoxin:<1.0EU/μg
- MolecularMass:11.0kDa
- Formulation:10mMSodiumAcetate,pH6.0
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
Determinedbythedose-dependentstimulationoftheproliferationofHUVECcells.
AASequence:
| GKKCIRTPKI | SKPIKFELSG | CTSMKTYRAK |
| FCGVCTDGRC | CTPHRTTTLP | VEFKCPDGEV |
| MKKNMMFIKT | CACHYNCPGD | NDIFESLYYR |
| KMYGDMA |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
