ProductDescription:
TGF-βfamilymembersarekeymodulatorsofcellproliferation,differentiation,matrixsynthesis,andapoptosis.Asimpliedbytheirname,BMPsinitiate,promote,andregulatethedevelopment,growth,andremodelingofboneandcartilage.Inadditiontothisrole,BMPsarealsoinvolvedinprenataldevelopmentandpostnatalgrowth,remodeling,andmaintenanceofavarietyofothertissuesandorgans.IncreasingevidenceindicatesthatBMP-Smadsignalinghasatumorsuppressingactivity,andthatBMPscaninhibittumorgrowth.BMP-6isabnormallyexpressedinbreastcancercelllines,however,itsfunctioninpromotingbreastcancerdevelopmentisunknown.ThematureandfunctionalformofBMP-6isahomodimeroftwoidentical139aminoacidpolypeptidechainslinkedbyasingledisulfidebond.EachmonomerisexpressedastheC-terminalpartofaprecursorpolypeptide,whichcontainsa20aminoacidsignalpeptideanda354aminoacidpropeptide.Thisprecursorundergoesintracellulardimerization,anduponsecretionitisprocessedbyafurin-typeprotease.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Activity:Typically0.03-0.06μg/mlED50
- Purity:≥95%
- Endotoxin:<1.0EU/μg
- MolecularMass:26.2kDa
- Formulation:10mMTris,pH9.0
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
DeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5cells.
AASequence:
| VSSASDYNSS | ELKTACRKHE | LYVSFQDLGW |
| QDWIIAPKGY | AANYCDGECS | FPLNAHMNAT |
| NHAIVQTLVH | LMNPEYVPKP | CCAPTKLNAI |
| SVLYFDDNSN | VILKKYRNMV | VRACGCH |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
