ProductDescription:
TGFbetafamilymembersarekeymodulatorsofcellproliferation,differentiation,matrixsynthesis,andapoptosis.Asimpliedbytheirname,BMPsinitiate,promote,andregulatethedevelopment,growth,andremodelingofboneandcartilage.Inadditiontothisrole,BMPsarealsoinvolvedinprenataldevelopmentandpostnatalgrowth,remodelingandmaintenanceofavarietyofothertissuesandorgans.BMP-3isabundantlyfoundinadultboneand,toalesserextent,fetalcartilage.BMP-3inhibitsosteogenesisandboneformationbyactivatingasignalingcascadethatantagonizesthesignalingofpro-osteogenicBMPs.RecombinantHumanBMP-3isadisulfide-linkedhomodimericproteinthatcorrespondstoresidues361to472ofthe472aminoacidBMP-3precursorprotein.
TechnicalSpecifications:
- Species:Human
- Expression:E.coliCellExpressed
- Purity:≥95%
- Endotoxin:<1.0EU/μg
- MolecularMass:25.2kDa
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
DeterminedbyitsABIlitytoinhibitBMP-2-inducedalkalinephosphataseproductionbyATDC-5cells.
AASequence:
MQWIEPRNCA | RRYLKVDFAD | IGWSEWIISP |
KSFDAYYCSG | ACQFPMPKSL | KPSNHATIQS |
IVRAVGVVPG | IPEPCCVPEK | MSSLSILFFD |
ENKNVVLKVY | PNMTVESCAC | R |
ReconstitutionBuffer:
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1-1.0mg/ml.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.