ProductDescription:
ADIponectinisanadipose-derivedsecretedproteincontaining226aminoacidresidues.Itisrelativelyabundantinhumansandrodents,accountingforabout0.01%oftotalplasmaprotein.Thecirculatinglevelsofadiponectinaredecreasedunderconditionsofobesity,insulinresistance,andtypeIIdiabetes.Disruptionofadiponectininmicecausesinsulinresistanceandneointimalformation.Conversely,admiNISTrationofrecombinantadiponectinsuppresseshepaticglucoseproduction,andreversesinsulinresistanceassociatedwithbothlipoatrophyandobesity.Theprotectiveroleofadiponectinisattributedtoitsanti-inflammatoryproperties(e.g.ABIlitytosuppressexpressionofTNF-αandclassAscavengerreceptorinmacrophages).RecombinantadiponectinisamultimericglycoproteincontainingaminoacidsGlu-19toAsn-244oftheadiponectinprecursorproteinfusedtoanN-terminalhistidinetag.
TechnicalSpecifications:
- Species:Human
- Expression:InsectCellExpressed
- Activity:Typically3.0-6.0μg/mlED50
- Purity:≥97%
- Endotoxin:<1.0EU/μg
- MolecularMass:25.9kDa
- Formulation:10mMTris,pH8.5+75mML-Arginine
- CountryofOrigin:USA
PurityConfirmation:
ThiswasdeterminedbySDS-PAGEgelandHPLCanalysis.
ActivityAssay:
DeterminedbyacytotoxicassayusingM1cells.
AASequence:
| RGHHHHHHHH | ETTTQGPGVL | LPLPKGACTG |
| WMAGIPGHPG | HNGAPGRDGR | DGTPGEKGEK |
| GDPGLIGPKG | DIGETGVPGA | EGPRGFPGIQ |
| GRKGEPGEGA | YVYRSAFSVG | LETYVTIPNM |
| PIRFTKIFYN | QQNHYDGSTG | KFHCNIPGLY |
| YFAYHITVYM | KDVKVSLFKK | DKAMLFTYDQ |
| YQENNVDQAS | GSVLLHLEVG | DQVWLQVYGE |
| GERNGLYADN | DNDSTFTGFL | LYHDTN |
ReconstitutionBuffer:
Reconstituteinwatertoaconcentrationof1.0mg/ml.Allowthereconstitutedvialtositatroomtemperaturefor1hourbeforeuse.Donotvortex.
Storage:
Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%HSA)andstoreinworkingaliquotsat-20°Cto-80°C.
