ProductDescription:
- Animal-derivedproductfree
- HighActivity
- LyophilizedandCarrierFree(CF)
Wnt3AHuXp isamemberoftheWntgenefamilythatconsistsofstructurallylinkedgenesthatencodesecretedsignalingproteins.Theseproteinsareassociatedwithoncogenesisandinnumerousdevelopmentalprocesses.Theseprocessesincluderegulationofcellfateandpatterningduringembryogenesis.HumanWnt3Aisaproteinwhichshows84%identityrelativetootherhumanWnt3proteins.Wnt3Aplaysakeyroleinmaintainingtheintegrityofembryonicandadulttissues.Thepost-translationalglycosylationandacylationofWntsareessentialfortheirefficientsecretionandBIOLOGicalactivity.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Activity:Typically≤20ng/mLEC50
- Purity:>90%
- Endotoxin:<1EU/μg
- MolecularMass:38-42kDa
- Formulation:1xPBSpH7.4+500mMNaCl+1%CHAPS
PurityConfirmation:
TheproteinwasresolvedbySDS-polyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.
ActivityAssay:
TheactivitywasdeterminedbydosedependentAlkalinePhosphataseproductioninMC3T3E1mousepreosteoblastcells(1)andstABIlizationofbetacatenininmouseL929cells(2).
ReconstitutionBuffer:
Brieflycentrifugethevialbeforeopening.Itisrecommendedtoreconstitutetheproteinto≤100μg/mLinsterile1xPBScontaining0.1%endotoxin-freerecombinanthumanserumalbumin(HSA).
References:
1)Walter,K.,andSchutt,C.,inMethodsofEnzymaticAnalysis,Bergmeyer,H.U.ed.,2nded.,VolumeII,AcademicPress,Inc.(NewYork,NY:1974),860-864.
2)vanNoortM,etal.,2002,JBiolChem,277(20),17901-5.
(ComplimentaryHSAwillbeprovidedwithshipment)
