ProductDescription:
- Endotoxin-free
- Animal-derivedproductfree
- LyophilizedandCarrierFree(CF)
- AuthenticGlycosylation
Xeno-freeIL-17FHuXpisahomodimericglycoproteinwithanapparentmolecularmassof38kDa.Thiscytokineisproducedinahumancellexpressionsystemwithserum-free,chemicallydefinedmedia.Productioninhuman293cellsoffersauthenticglycosylation,whichcontributestostABIlityincellgrowthmediaandotherapplications.IL-17iscommonlyassociatedwithallergicresponses.IL-17inducestheproductionofmanyothercytokines(suchasIL-6,G-CSF,GM-CSF,IL-1beta,TGFbeta,andTNFalpha),chemokines(includingIL-8,GROalpha,andMCP-1),andprostaglandins(e.g.,PGE2)frommanycelltypes(fibroblasts,endothelialcell,epithelialcells,keratinocytes,andmacrophages).Thereleaseofcytokinescausesmanyfunctions,suchasairwayremodeling,acharacteristicofIL-17responses.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Activity:Typically≤10ng/mLEC50
- Purity:>95%
- Endotoxin:<1EU/µg
- MolecularMass:38kDa,homodimer,glycosylated
- Formulation:1xPBS
PurityConfirmation:
TheproteinwasresolvedbySDS-polyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.
ActivityAssay:
Theactivitywasdeterminedbythedose-dependentinductionofIL-6secretionfromNHDFAdultfibroblasts.
ReconstitutionBuffer:
Brieflycentrifugethevialbeforeopening.Itisrecommendedtoreconstitutetheproteininsterile1xPBScontaining0.1%endotoxin-freerecombinanthumanserumalbumin(HSA).
