ProductDescription:
- Animal-derivedproductfree
- HighActivity
- LyophilizedandCarrierFree(CF)
- Glycosylated
Interferongamma(IFNγ),isacytokinealsoknownastypeIIinterferonthatprovidesimmunityagainstbacterial,viralandprotozoaninfections.Initsactiveform,IFNγoccursasaglycosylated,non-covalentlylinkedhomodimerof29-32kDasubunits.Itisproducedbyanumberofimmunecelltypessuchasthenaturalkillercells,naturalkillerTcells,andeffectorlymphocyteTcellsfollowingantigenicandinflammatorytriggers.Followingsecretion,theIFNγdimerbindstoitsreceptorwhichhastwosubunits:IFN-γR1whichistheligand-bindingchain(αchain)andIFN-γR2,thesignal-transducingchain(βchain).TheactivationoftheIFNγreceptorleadstoactivationoftheJAK/STATpathwaywhichinturnactivatesIFNγresponsivegenes.WhileIFNγcaninhibitviralreplication,italsoworksasanimmune-modulatorandimmune-stimulatorbyincreasingsurfaceexpressionofclassIMHCproteins.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Activity:Typically≤0.05ng/mLEC50
- Purity:>95%
- Endotoxin:<1EU/μg
- MolecularMass:29-32kDa
- Formulation:1xPBS
PurityConfirmation:
TheproteinwasresolvedbySDS-polyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.
ActivityAssay:
TheactivitywasdeterminedbydosedependentinhibitionofproliferationofHT-29cells.
Reference:
- BilliauA&MatthysP.(2009)Interferon-gamma:ahistoricalperspective.CytokineGrowthFactorRev20(2):97–113.
- SchroderKetal.(2004)Interferon-gamma:anoverviewofsignals,mechanismsandfunctions.JLeukocBiol75(2):163–89
ReconstitutionBuffer:
Brieflycentrifugethevialbeforeopening.Itisrecommendedtoreconstitutetheproteininsterile1xPBScontaining0.1%endotoxin-freerecombinanthumanalbumin(HSA).
