ProductDescription:
- Endotoxin-free
- Animal-derivedproductfree
- AvailableinBulk
- LyophilizedandCarrierFree(CF)
- Glycosylateddisulfide–linkedhomodimer
Xeno-freeBMP-7HuXpisexpressedinhuman293cellsasadisulfidelinkedhomodimericglycoproteinwithanapparentmolecularmassof29kDa.Thiscytokineisproducedinaserum-free,chemicallydefinedmedia.RecombinantHumanBMP-7isahomodimericglycoproteinconsistingoftwo117aminoacidsubunits,whichcorrespondtoaminoacidresidues315to431ofthefull-lengthBMP-7precursor.TheproteinencodedbythisgeneisamemberoftheTGF-betasuperfamily.LikeothermembersoftheBoneMorphogeneticProteinfamilyofproteins,itplaysakeyroleinthetransformationofmesenchymalcellsintoboneandcartilage.
TechnicalSpecifications:
- Species:Human
- Expression:HEK293CellExpressed
- Activity:Typically≤100ng/mLEC50
- Purity:>95%
- Endotoxin:<1EU/µg
- MolecularMass:29kDa,homodimer,glycosylated
- Formulation:2xPBS+20%Ethanol
PurityConfirmation:
TheproteinwasresolvedbySDS-polyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.
ActivityAssay:
TheactivitywasdeterminedbythedosedependentinductionofalkalinephosphataseproductionintheATDC-5cellline(Mousechondrogeniccellline).
ReconstitutionBuffer:
Brieflycentrifugethevialbeforeopening.Itisrecommendedtoreconstitutetheproteininsterilewatercontaining0.1%endotoxin-freerecombinanthumanserumalbumin(HSA).