
ProductDescription:
- Produceshighqualitydendriticcells
- Yieldsareequaltoorbetterthanstandardprotocols
- Nomediumchangeoradditionrequired
- Nocytokinealiquotingrequired
- XenoandAnimal-derivedcomponentfree
- Humancellexpressedgrowthfactorsincluded
HumanKine®G4DendriticCell(DC)GenerationKithasbeendevelopedforthegrowthandgenerationofhumandendriticcells(DCs).UsingHumanKineG4DCmedium,humandendriticcellscanbeproducedfromperipheralbloodmononuclearcellsWITHOUTMEDIUMCHANGEandusedeitherasimmatureDCsorfurthercultivatedintomatureDCs.
ProtocolinBrief:
- ReconstituteCytokineCocktail
- AddCocktailtoBasalMedium
- SUSPendmonocytesincompletemedium
- Incubate7days
- DONOTCHANGEMEDIUM
- DONOTADDMEDIUM.
Linktocompleteprotocol
Specifications:
KitContents:
G4DCBasalMedium
GrowthFactorCocktail
Protocol
Kitincludesallnecessarymediumandreagents(exceptbeta-mercaptoethanolandFetalCalfSerum)
PackSize:
100mLHZ-9101
500mLHZ-9501
NOTE:A10mLculturerequiresonly10mLmedium.(plating3to5x105cells/mL)Nomediumadditionorchangeisrequired.
StorageandStABIlity:
G4DCBasalMedium:Storeat4ºC,protectfromlight.Mediumisstableforsixmonthsfromdateofproduction.
G4DCCocktail(Lyophilized):Storeat-80ºC.Cocktailisstablefortwelvemonthsfromdateofreceipt.
Completemedium(G4DCCocktailaddedtoG4DCBasalMedium):Canbestoredat4ºCforupto30daysinthedark.
QualityConfirmation:
Acertificateofanalysisisavailableonrequest.
ProductUse:
AddingtheGrowthFactorCocktailtotheG4DCBasalMediumproducesG4DendriticCellGenerationMediumrequiringnomediumadditionorchangeduringtheincubationperiod.
Theresultingcellsaremonocyte-derivedimmatureDCsthatarehighlyphagocyticwiththeimmatureDCsurfaceexpressionphenotype(CD14neg,CD83neg,CD80llow,CD86low,MHCIint,andMHCint)andcanmigrateinresponsetoCCR5agoNIST(RANTES),butnottoCCR7agonists(ELC/SLC).
PurityConfirmation:
TheproteinwasresolvedbySDSpolyacrylamidegelelectrophoresisandthegelwasstainedwithCoomassieblue.